Journal: Pharmaceutics

Article Title: Self-Stabilizing Covalent Ligand Targets Bacterial Phosphatidylethanolamine and Enhances Antibiotic Efficacy

doi: 10.3390/pharmaceutics18010071

Figure Lengend Snippet: ( a ) 13 C-NMR spectra of single compounds and mixtures of DMAX and OPE, OXA and OPE, or DMAO and OPE. ( b ) FT-IR spectra of the single compounds and mixtures of DMAX and OPE, OXA and OPE, DMAO and OPE. The key peak of Schiff base is marked with an arrow. ( c ) 13 C-NMR spectra of single compounds and mixtures of DMAX and OA, OXA and OA, or DMAO and OA. ( d ) FT-IR spectra of single compounds and mixtures of DMAX and OA, OXA and OA, or DMAO and OA. ( e ) 13 C-NMR spectra of DMAX plus OPE with NaCl (500 mM). The spectrum of the mixture at 0 mM NaCl was used as control. Dotted squares indicate the region of the ketone group in 13 C-NMR spectra and the imine bond in FT-IR spectra. ( f ) Preparation of lipid-coated wells to study the binding of fluorescein and DMAX-FITC. ( g ) Relative fluorescence intensities of DMAX-FITC on different membranes normalized to uncoated wells. The fluorescein result was subtracted to obtain the difference. The data are mean ± standard error of mean (SEM) ( n = 8); p value calculated by Student’s t -test.

Article Snippet: Fourier-transform infrared spectroscopy (FT-IR) was performed using an FT/IR-6300 (JASCO Corporation, Tokyo, Japan).

Techniques: Control, Binding Assay, Fluorescence